Composite

Part:BBa_K2505007:Design

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)


Pcps-rbs-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter used in this part is derived from E. coli and acativated through histidine-to-aspartate phosphorelay. A His-to-Asp phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. One of the His-to-Asp phosphorelay systems in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, and RcsB, a response regulator. This system is activated after stress exposure such as osmolality shock; cps operon promoter (which controls the production of polysaccharides) is induced through the RcsC→RcsD→RscB→cps pathway.


Source

Pcps-rbs was derived from E. coli.

References

Suzuki, T., Miwa, K., Ishikawa, K., Yamada, H., Aiba, H. and Mizuno, T. (2001) The Arabidopsis Sensor His-kinase, AHK4, Can Respond to Cytokinins. Plant Cell Physiol. 42: 107-113.

Spíchal, L., Rakova, N.Y., Riefler, M., Mizuno, T., Romanov, G.A.,Strnad, M. and Schmülling, T. (2004) Two Cytokinin Receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, Differ in their Ligand Specifity in a Bacterial Assay. Plant Cell Physiol. 45: 1299-1305.